The Selecao captain missed training on Monday after suffering 10 fouls in the 1-1 draw with Switzerland, and ended up limping out on Tuesday.
Brazil star Neymar hobbled out of training with Brazil ahead of their Group E clash with Costa Rica on Friday.
Neymar was absent from training on Monday, missing the session a day after Brazil drew 1-1 with Switzerland in their opening World Cup clash.
The Paris Saint-Germain forward received some tough treatment from Switzerland, being fouled 10 times during the game, his first competitive start since February.
A fractured foot kept Neymar out of the end of PSG's season but he made a goalscoring return to action with a stunning solo strike against Croatia in a friendly at Anfield, and was also on target in a 3-0 warm-up victory against Austria.
Tite's men looked set to receive a boost with Neymar taking part in Tuesday's session.
But after around 10 minutes he limped away with a member of staff to receive some treatment after looking clearly uncomfortable on the field.
After Sunday's match, Switzerland coach Vladimir Petkovic denied that his side intentionally fouled Neymar, saying: "Most of the duels were perfectly clean and very often we had one-on-ones with Neymar... I didn't see any bad fouls. My team played it very cleverly. We did some good things and I'm pleased."
The coach's words were backed up by Swiss star Stephan Lichtsteiner, who was worried the referee would intervene: “It’s very difficult playing him. He’s fast, he’s technical, you need to keep up with him all the time because he’s dangerous. It’s a big challenge. And I was a little bit worried about the referee. Every time he fell down it was a whistle, a foul, so that was a bit of a challenge.
“You think to yourself 'don’t be too aggressive' because every time he goes down it’s a foul. So, because of that, I had to be a bit careful.”
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